Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags
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چکیده
منابع مشابه
Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags
We report oriented immobilization of proteins using the standard hexahistidine (His6)-Ni2+:NTA (nitrilotriacetic acid) methodology, which we systematically tuned to give control of surface coverage. Fluorescence microscopy and surface plasmon resonance measurements of self-assembled monolayers (SAMs) of red fluorescent proteins (TagRFP) showed that binding strength increased by 1 order of magni...
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Corresponding author: Jae-Hang Shim, M.D., Ph.D., Department of Anesthesiology and Pain Medicine, Hanyang University Hospital, 249-1, Gyomun-dong, Guri 471-701, Korea. Tel: 82-31-560-2390, Fax: 82-31-563-1731, E-mail: [email protected] This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by...
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ژورنال
عنوان ژورنال: ACS Nano
سال: 2017
ISSN: 1936-0851,1936-086X
DOI: 10.1021/acsnano.7b03717